Direct 1,3-butadiene biosynthesis in Escherichia coli via a tailored ferulic acid decarboxylase mutant

Abstract The C4 unsaturated compound 1,3-butadiene is an important monomer in synthetic rubber and engineering plastic production. However, microorganisms cannot directly produce when glucose used as a renewable carbon source via biological processes. In this study, we construct artificial metabolic pathway for production from Escherichia coli by combining the cis,cis -muconic acid ( cc MA)-producing together with tailored ferulic decarboxylase mutations. rational design of substrate-binding site enzyme computational simulations improves MA decarboxylation thus We find that changing dissolved oxygen (DO) levels controlling pH are factors Using DO–stat fed-batch fermentation, 2.13 ± 0.17 g L ?1 1,3-butadiene. results indicate can unnatural/nonbiological compounds strategy.